Recipient Inclusion Criteria:
- Age ≥ 18 years (no upper age limit, but physician discretion is advised)
- AML that is refractory to 2 courses of induction therapy (that together constitute the 'first-line' therapy) or that has relapsed after a period of morphologic complete remission or morphologic remission with incomplete blood count recovery (CRi)
- Candidacy for intense induction chemotherapy (ECOG 0-2, adequate renal, liver and cardiac function, absence of uncontrolled infections)
- Availability of parents, siblings or children who are HLA haploidentical (and not homozygous for the shared haplotype), who are deemed suitable donors after medical evaluation, and who complete peripheral blood mononuclear cell collection
- No history of autologous or allogeneic stem cell transplant, purine analog chemotherapy or cyclophosphamide, or total body irradiation
- Ability to comprehend the investigational nature of the study and provide informed consent
Recipient Exclusion Criteria:
- Acute promyelocytic leukemia (including those with non-classical rearrangements of RARα)
- History of severe myelodysplastic syndrome clearly preceding the diagnosis of AML (i.e., red cell transfusion dependence or erythropoietin dependence over a 4-month period, or in the absence of a clear cause, any of the following: hemoglobin consistently below 9 g/dL or platelets below 50 x 10\^9/L or ANC below 1000/uL on 2 or more occasions 2 weeks apart, or use of G-CSF to maintain the ANC threshold in the absence of infection, in the 3 months preceding the diagnosis of AML). Exception: If ATAC therapy is being considered as a bridge to stem cell transplantation in patients with an available standard transplant donor (familial, unrelated, or cord blood), this exclusion criterion does not apply.
- Grade 2-3/3 fibrosis in the diagnostic bone marrow biopsy
- DLCO < 40% predicted
- Left ventricular ejection fraction < 40% (evaluated by ECHO or MUGA)
- AST/SGOT > 2.5 x ULN
- Bilirubin > 1.5 x ULN
- Creatinine > 1.5 x ULN
- Creatinine clearance < 50 mL/min
- HIV positive
- Major anticipated illness or organ failure incompatible with survival from chemotherapy
- Concurrent second primary cancer or a prior malignancy that required cytotoxic treatment within the past 12 months, other than cervical carcinoma in-situ or prostate cancer in-situ
- Severe psychiatric illness or mental deficiency sufficiently severe as to make compliance with the treatment unlikely and informed consent impossible
- Any congenital or acquired immunodeficiency that would possibly permit permanent engraftment of donor cells
- Receiving systemic steroid therapy or systemic immunosuppression such as cyclosporine or TNF-inhibitors
- Prior or concurrent receipt of any marketed or investigational agent deemed on an ad hoc basis to cause immunomodulation, pose a threat of permanent engraftment or increase the risk of GVHD.
Donor inclusion criteria:
- Mismatched family donor (incompatibility at 3 loci HLA-A, B and DR of the unshared haplotype, or higher-order incompatibility)
- Age ≥ 16 and ≤ 80 years
- Fit to undergo apheresis (normal blood counts, normotensive and no history of stroke).
- Donor has been tested negative for HIV-1, HIV-2, hepatitis B virus (HBV, surface and core antigen), hepatitis C virus, human T-lymphotropic virus types I/II, and Treponema pallidum (syphilis).
- ECOG performance status of 2 or less.
- Adequate veins for leukapheresis or agree to placement of a temporary central venous catheter.
- Donor must provide written informed consent.
- Where multiple equally-suitable donors are available, sex mismatched donors will be preferred.
Donor exclusion criteria:
- Medically uncontrolled coronary heart disease
- Myocardial infarction within the last 3 months
- History of seizure
- History of stroke
- History of malignancy (except basal cell or squamous carcinoma of the skin, or positive PAP smear and subsequent negative follow-up)
- Presence of a transmissible disease (such as HIV seropositivity)
- Presence of a major illness or a suspected systemic dysfunction
- Presence of an an active inflammatory or autoimmune disorder
- Female donors who are pregnant or nursing