Recruiting
Phase 2

TRT & LT

Sponsor:

North Florida Foundation for Research and Education

Code:

NCT04460872

Conditions

Spinal Cord Injury

Spinal Cord Injuries

Trauma, Nervous System

Wounds and Injury

Central Nervous System Diseases

Eligibility Criteria

Sex: Male

Age: 18+

Healthy Volunteers: Not accepted

Interventions

Testosterone Enanthate

Locomotor Training

Study Details

Brief summary:

This pilot study will determine the feasibility of implementing a combinatory rehabilitation strategy involving testosterone replacement therapy (TRT) with locomotor training (LT; walking on a treadmill with assistance and overground walking) in men with testosterone deficiency and walking dysfunction after incomplete or complete spinal cord injury. The investigators hypothesize that LT+TRT treatment will improve muscle size and bone mineral density in men with low T and ambulatory dysfunction after incomplete or complete SCI, along with muscle fundtion and walking recovery in men with T low and ambulatory dysfunction ater incomplete SCI.

Conditions

Spinal Cord Injury

Spinal Cord Injuries

Trauma, Nervous System

Wounds and Injury

Central Nervous System Diseases

Study ID

NCT04460872

Start date

Jan 31, 2021

Status verified date

May, 2025

Completion date

Jun 30, 2026

Anticipated

Primary completion date

Jan 30, 2026

Anticipated

Eligibility Criteria

Eligibility Criteria

Sex: Male

Age: 18+

Healthy Volunteers: Not accepted

Inclusion Criteria:

  • Men >18 years of age
  • Diagnosis of an incomplete SCI involving spinal segments L1 or above or a clinically complete SCI involving spinal segments T2-L1, with upper motor neuron injury signs (i.e., spasticity, hypertonicity) for >60-days
  • Low serum total testosterone (<300 ng/dL), bioavailable testosterone (<110 ng/dL), or free testosterone (<46 pg/mL or <4.6 ng/dL)
  • Presence of one or more sign or symptom that may be related to low testosterone, including: loss of body hair or reduced shaving, very small testes (<6 mL), reduced sexual desire (libido) and activity, decreased spontaneous erections (e.g., morning erections) or erectile dysfunction, breast discomfort or gynecomastia, height loss, low-trauma fracture, or low BMD, hot flushes or sweats, decreased energy, motivation, initiative, or self-confidence, fatigue or irritability, feeling sad or blue, having a depressed mood, or having a persistent low-grade depressive disorder, poor concentration or memory, sleep disturbances or increased sleepiness, mild unexplained anemia (normochromic or normocytic), reduced muscle bulk, strength, or physical performance, Increased body fat or body mass index, any other sign or symptom commonly associated with low testosterone
  • Locomotor dysfunction, definted as self-selected walking pace ≤1.0 m/s on a 10mWT, either with or without gait devices or braces and with or without assistance, or as self-selected walking pace >1.0 m/s with reliance on a gait device or brace or with highly compensated movement impairments, as identified by a trained observer.
  • Diagnosis of first time SCI including etiology from trauma, vascular, or orthopedic pathology
  • Medically-stable condition that is asymptomatic for conditions that will interfere with the study participation
  • Willingness to administer TRT as instructed by the study staff and to abide by study protocol
  • Documented approval from the study physician verifying medical status

Exclusion Criteria:

  • Currently participating in another research protocol that may influence study outcomes.
  • Mental state that precludes understanding the study protocol.
  • Life expectancy <12-months.
  • History of or current congenital SCI (e.g., Chiari malformation, myelomeningocele, intraspinal neoplasm, Frederich's ataxis) or other degenerative spinal disorder (e.g., spinocerebellar degeneration) that may complicate study procedures
  • Multiple sclerosis, amyotrophic lateral sclerosis, or other neurologic impairment or injury
  • Current prostate, breast, or other organ cancer or a history of prostate or breast cancer
  • Any other diagnosed or treated cancer within the past 24-months, with the exceptions of basal or squamous cell carcinoma of the skin that has been successfully treated
  • Serum prostate-specific antigen (PSA) >3.0 ng/mL \[men treated with 5-alpha reductase inhibitors (e.g., finasteride or dutasteride) are eligible to participate if PSA values are ≤1.5 ng/mL\]
  • Prostate nodule or induration noted on digital rectal exam (DRE) during screening that tests positive for prostate cancer
  • Currently seeking fertility or expected during the duration of the study
  • Gynecomastia
  • Hematocrit (HCT) >49%
  • Any major cardiovascular (CV) event within the last 12-months (defined as a history of acute myocardial infarction, any cardiac revascularization procedure including angioplasty, stenting, or coronary artery bypass grafting, revascularization of the carotid or middle cerebral artery or procedures to treat critical limb ischemia, or hospitalization due to unstable angina, transient ischemic attack, stroke, or peripheral vascular disease)
  • Angina that is not controlled on a current medical regimen (Canadian class II, III, or IV)
  • Poorly compensated congestive heart failure (NYHA class III or IV)
  • Poorly controlled hypertension (consistently measured systolic BP ≥160 mmHg or diastolic BP ≥100 mmHg), while on medications
  • Poorly controlled arrhythmia of any type
  • Severe valvular heart disease
  • Baseline electrocardiogram (ECG) findings such as left bundle branch block or marked ECG abnormalities that would preclude serial screening evaluations for occult ischemic events
  • History of unprovoked deep venous thrombosis (DVT), unprovoked pulmonary embolism, history of recurrent DVT or known thrombophilia
  • LDL cholesterol >160 mg/dL with history of any major CV event, defined above, within the last 12-months
  • Major non-CV surgery (e.g., major abdominal or thoracic procedure) within 90-days prior to screening and/or a major surgery scheduled at the time of screening
  • Liver enzymes (AST or ALT) >1.5 times the normal upper limit
  • Severe or end-stage chronic kidney disease documented by estimated glomerular filtration rate (eGFR) <30 mL/min
  • Diagnosed, but untreated severe obstructive sleep apnea
  • Lower extremity fracture in the last 12-months (exclusion criterion for participation in LT+TRT group only)
  • Femoral neck, total hip, or lumbar spine t-score below -2.5 or distal femur BMD <0.70 g/cm2, assessed via DEXA at screening (exclusion criterion for participation in LT+TRT group only)
  • Current anticoagulant therapy (contraindication for i.m. injections)
  • Use of any of the following pharmacologic agents in the previous 90-days: any TRT formulation, any compounded or over-the-counter androgenic hormones or androgen precursors, clomiphene, aromatase inhibitors, anti-estrogen or estrogen treatment, or growth hormone
  • Use of anti-resorptive or bone anabolic drug therapy in the previous 180-days
  • Acute use (>5-days) of any opioids (e.g., oxycodone, hydrocodone, etc) or systemic glucocorticoids >7.5 mg/d prednisone equivalent (e.g., hydrocortisone 30 mg, methylprednisolone 6 mg, or dexamethasone 1.2 mg) within 1-week before screening visit, except men who are taking these medications for a chronic condition and are anticipated to continue treatment for the study duration
  • Known allergy to any component of the TRT formulation (e.g., sesame oil or cottonseed oil)
  • Any other condition, therapy, lab abnormality, medical or psychiatric conditions, or reason that might pose a risk to the participant, make participation not in the person's best interest, confound the study results (e.g., inability to comply with study requirements), make the participant unsuitable to receive study intervention, or interfere with the person's ability to participate for the entire study duration

Study Design

Enrollment

21 participants

Anticipated

Allocation

Non randomized

Intervention Model

Parallel Assignment

Primary purpose

Supportive Care

Interventions and Outcome Measures

Arms

experimental: testosterone enanthate

Testosterone enanthate via i.m. injection (100 mg/week)

experimental: locomotor training, testosterone enanthate

Treadmill and overground walking training and testosterone enanthate via i.m. injection (100 mg/week)

no intervention: non-interventional control

Non-interventional control group

Interventions

Testosterone Enanthate

Subjects receive testosterone (100 mg/week) by intramuscular injection

Locomotor Training

Subjects receive locomotor training (4 sessions/week for 2-3 months)

Primary outcome measure

  • change in thigh muscle cross-sectional area [ Time Frame: baseline, 3 months, 6 months ]
  • change in 6 min walk test (6MWT) [ Time Frame: baseline, 1 month, 3 months, 6 months ]
  • change in distal femur bone mineral density [ Time Frame: baseline, 3 months, 6 months ]

Central Contacts and Locations

Central contacts

Joshua F Yarrow, PhD

(352) 548-6477joshua.yarrow@va.gov

Dana M Otzel, PhD

(352) 548-6477dana.otzel@va.gov

Locations

North Florida/South Georgia Veterans Health System

Recruiting

Gainesville, Florida, United States, 32608

Contacts

Dana M Otzel, PhD

(352) 548-6477dana.otzel@va.gov

Joshua F Yarrow, PhD

joshua.yarrow@va.gov

Brooks Rehabilitation

Recruiting

Jacksonville, Florida, United States, 32216

Contacts

Emily J Fox, DPT, PhD

(352) 273-6117ejfox@phhp.ufl.edu

More Information

Sponsor

North Florida Foundation for Research and Education

Last update posted

May 6, 2025

Last verified

May, 2025

Keywords

  • Testosterone
  • Testosterone enanthate
  • Testosterone undecanoate
  • Testosterone 17 beta-cypionate
  • Methyltestosterone
  • Androgens
  • Hormones
  • Hormone Substitutes, and Hormone Antagonists
  • Physiologic Effects of Drugs
  • Pharmacologic Actions
  • Therapeutic Uses
  • Anabolic Agents
  • Testosterone Replacement Therapy
  • Dual Energy X ray Absorptiometry
  • Lean Tissue Mass
  • Body Composition
  • Muscle Strength
  • Muscle Mass
  • Bone Mineral Density
  • Adipose Tissue
  • Body Fat
  • Density, Bone
  • Bone Formation
  • Bone Resorption
  • Bone Density Conservation Agents
  • Magnetic Resonance Imaging
  • Walking
  • Ambulation
  • Locomotor
  • Locomotion
  • Treadmill
  • Spinal Cord Injury

Trial information was received from ClinicalTrials.gov and was last updated on 2026-09-10. This information was provided to ClinicalTrials.gov by North Florida Foundation for Research and Education on 2025-05-06.