Recruiting
Phase 1

ONC206

Sponsor:

Sabine Mueller, MD, PhD

Code:

NCT04732065

Conditions

Diffuse Midline Glioma (DMG)

Glioblastoma

Recurrent Ependymoma

Recurrent Malignant Central Nervous System Neoplasm

Spinal Cord Glioma

Eligibility Criteria

Sex: All

Age: 2 - 21

Healthy Volunteers: Not accepted

Interventions

ONC206

Standard of Care Radiation Therapy

Optional Proton (1H) MR spectroscopy (MRS)

Study Details

Brief summary:

This phase I trial studies the effects and best dose of ONC206 alone or in combination with radiation therapy in treating patients with diffuse midline gliomas that is newly diagnosed or has come back (recurrent) or other recurrent primary malignant CNS tumors. ONC206 is a recently discovered compound that may stop cancer cells from growing. This drug has been shown in laboratory experiments to kill brain tumor cells by causing a so called "stress response" in tumor cells. This stress response causes cancer cells to die, but without affecting normal cells. ONC206 alone or in combination with radiation therapy may be effective in treating newly diagnosed or recurrent diffuse midline gliomas and other recurrent primary malignant CNS tumors.

Conditions

Diffuse Midline Glioma (DMG)

Glioblastoma

Recurrent Ependymoma

Recurrent Malignant Central Nervous System Neoplasm

Spinal Cord Glioma

Study ID

NCT04732065

Start date

Aug 23, 2021

Status verified date

Jun, 2026

Completion date

Jul 31, 2027

Anticipated

Primary completion date

May 31, 2027

Anticipated

Eligibility Criteria

Eligibility Criteria

Sex: All

Age: 2 - 21

Healthy Volunteers: Not accepted

Inclusion Criteria:

  • ARM A: Children and young adults with DMG, H3K27 altered (Dose escalation: 2-21 years of age; Dose expansion: 2 years of age and above) who completed at least one line of prior therapy. Prior treatment must have included focal radiation therapy and patients must be within 4-14 weeks from completion of radiation therapy to registration (patients must start treatment within 1 week from registration), have not started any other therapies post-radiation, and have no evidence of disease progression.
  • ARM A: Tumor tissue confirmation of DMG, H3K27 altered is mandatory and pathology must be consistent with a DMG, H3K27 altered.
  • ARM A: Participants must have recovered from all acute side effects of prior therapy.
  • ARM A: From the projected start of scheduled study treatment, the following time periods must have elapsed: 5 half-lives from any investigational agent, 4 weeks from cytotoxic therapy (except 23 days for temozolomide and 6 weeks from nitrosoureas), 4 weeks from antibodies and must be at least 7 days since the completion of therapy with a biologic or small molecule agent. For any biologic or small molecule agent with known adverse events that can occur beyond 7 days after administration, the period prior to enrollment must be beyond the time during which adverse events are known to occur (these should be discussed with the study team)
  • ARM B: Newly diagnosed children and young adults (Dose escalation: 2-21 years of age; Dose expansion: 2 years of age and above) with a diagnosis of DMG, H3K27 altered are eligible, including spinal cord DMGs.
  • ARM B: Tumor tissue confirmation of DMG is mandatory and pathology must be consistent with a DMG, H3K27 altered.
  • ARM C: Children and young adults with DMGs (Dose escalation: 2-21 years of age; Dose expansion: 2 years of age and above) who have evidence of progression but have not been treated for this progression and are recommended to get re-irradiation.
  • ARM C: Patients must have undergone prior focal radiation therapy as part of their initial therapy and should be at least 6 months from prior radiation therapy. If timing is less than 6 months from prior focal radiation, these patients need to be discussed with the study chair(s).
  • ARM C: Tumor tissue confirmation is mandatory and pathology must be consistent with a DMG, H3K27 altered.
  • ARM C: Participants must have recovered from all acute side effects of prior therapy
  • ARM C: From the projected start of scheduled study treatment, the following time periods must have elapsed: 5 half-lives from any investigational agent, 4 weeks from cytotoxic therapy (except 23 days for temozolomide and 6 weeks from nitrosoureas), 4 weeks from antibodies and must be at least 7 days since the completion of therapy with a biologic or small molecule agent. For any biologic or small molecule agent with known adverse events that can occur beyond 7 days after administration, the period prior to enrollment must be beyond the time during which adverse events are known to occur (these should be discussed with the study team)
  • ARM D: Children and young adults with recurrent primary malignant CNS tumors, excluding DMGs, (Dose escalation: 2-21 years of age; Dose expansion: 2 years of age and above) who have evidence of progression but have not been treated for this progression. Participants who received a surgical resection for that progression are eligible if surgery has no curative intent. These patients need to be discussed with the study team.
  • ARM D: Prior tumor tissue confirmation is mandatory and pathology from the primary tumor must be consistent with malignant CNS tumor (diagnosis of ependymoma is allowed).Tissue at the time of progression is not required.
  • ARM D: Participants must have recovered from all acute side effects of prior therapy
  • ARM D: From the projected start of scheduled study treatment, the following time periods must have elapsed: 5 half-lives from any investigational agent, 4 weeks from cytotoxic therapy (except 23 days for temozolomide and 6 weeks from nitrosoureas), 4 weeks from antibodies and must be at least 7 days since the completion of therapy with a biologic or small molecule agent. For any biologic or small molecule agent with known adverse events that can occur beyond 7 days after administration, the period prior to enrollment must be beyond the time during which adverse events are known to occur (these should be discussed with the study team). Bevacizumab used for pseudoprogression does not require a wash out period.
  • TARGET VALIDATION: Newly diagnosed children and adults (2 years of age and above) with imaging consistent with a DMG, H3K27 altered are eligible.
  • TARGET VALIDATION: Children and young adults with recurrent primary malignant CNS tumors, including recurrent DMG, (2 years of age and above) who have evidence of progression but have not been treated for this progression.
  • TARGET VALIDATION: Participants must undergo tumor tissue collection as part of their standard of care
  • Participants who are receiving steroids must be on a stable or decreasing dose for at least 3 days prior to registration.
  • Peripheral absolute neutrophil count (ANC) >= neutrophil 1.0 g/l.
  • Platelet count >= 100 x 10\^9/L (transfusion independent, defined as not receiving platelet transfusions for at least 7 days prior to enrollment).
  • Serum creatinine < 1.5 Upper Limit normal (ULN) based on age and gender.
  • Total bilirubin <= 1.5 x upper limit of normal (ULN) for age; in presence of Gilbert's syndrome, total bilirubin < 3 x ULN or direct bilirubin < 1.5 x ULN.
  • Alanine aminotransferase (ALT) <= 3 x ULN.
  • Aspartate aminotransferase (AST) <= 3 x ULN.
  • Patients with seizure disorder may be enrolled if seizure disorder is well controlled
  • The effects of ONC206 on the developing human fetus is unknown. For this reason, females of child-bearing potential and males must agree to use adequate contraception. Adequate methods include: hormonal or barrier method of birth control; or abstinence prior to study entry and for the duration of study participation. Should a woman become pregnant or suspect she is pregnant while she or her partner is participating in this study, she should inform her treating physician immediately. Males treated or enrolled on this protocol must also agree to use adequate contraception prior to the study and for the duration of study participation
  • Karnofsky >= 50 for participants > 16 years of age and Lansky >= 50 for participants =< 16 years of age. Participants who are unable to walk because of paralysis, but who are up in a wheelchair, will be considered ambulatory for the purpose of assessing the performance score
  • Participants must be willing to provide adequate tissue. A minimum of 10-20 paraffin embedded unstained slides OR 1 block with tumor content of 40% or greater is required. Frozen tissue is also acceptable. Participants who previously enrolled on PNOC022 and provided adequate tissue, may not need to submit additional tissue - confirm with Study Chairs. Participants who do not meet this criterion may be discussed on a case-by-case basis with the Study Chairs.
  • A legal parent/guardian or participant must be able to understand, and willing to sign, a written informed consent and assent document, as appropriate
  • Patients must be enrolled on PNOC COMP if PNOC COMP is open to accrual at the enrolling institution

Exclusion Criteria:

  • Arm A \& B: For tumors that do not have a pontine or spinal cord epicenter the following specific exclusion criteria apply: Thalamic DMG and cerebellar, H3K27 altered that has undergone standard radiation without concurrent therapy (other than temozolomide).
  • Arm C \& D: Patients who participated in trials investigating ONC201 in the upfront setting will not be eligible. Prior ONC201 exposure as part of PNOC022 or expanded access programs will be allowed.
  • Participants who are currently receiving another investigational drug are not eligible.
  • Participants who are currently receiving other anti-cancer agents are not eligible.
  • Participants with a known disorder that affects their immune system, such as human immunodeficiency virus (HIV) or hepatitis B or C, or an auto-immune disorder requiring systemic cytotoxic or immunosuppressive therapy are not eligible. Note: Participants that are currently using inhaled, intranasal, ocular, topical or other non-oral or non-intravenous (IV) steroids are not necessarily excluded from the study but need to be discussed with the study chair.
  • Participants with uncontrolled infection.
  • Female participants of childbearing potential must not be pregnant or breast-feeding. Female participants of childbearing potential must have a negative serum or urine pregnancy test prior to the start of therapy.
  • Active illicit drug use or diagnosis of alcoholism.
  • History of allergic reactions attributed to compounds of similar chemical or biologic composition to ONC206.
  • Inability to follow the procedures of the study, e.g. due to language problems, psychological disorders, dementia, etc. of the participant or family.
  • Any participants with illnesses that may affect absorption of ONC206.
  • Any participants on strong inhibitors or inducers of CYP3A4, 2D6, 1A2, 2C9 and 2C19 at least 14 days prior and throughout the study.

Study Design

Enrollment

208 participants

Anticipated

Allocation

Non randomized

Intervention Model

Parallel Assignment

Primary purpose

Treatment

Interventions and Outcome Measures

Arms

experimental: Arm A: ONC206 for participants with diffuse midline gliomas + prior therapy

Participants receive ONC206 orally (PO) up to six times per week. Cycles repeat every 28 days for up to 12 months in the absence of disease progression or unacceptable toxicity. In case the participant receives clinical benefit from the treatment, treatment can proceed up to 24 months.

experimental: Arm B: ONC206 + radiation therapy for newly diagnosed participants

Participants undergo standard of care radiation therapy daily 5 days a week and receive ONC206 PO up to six times per week. Cycles repeat every 28 days for up to 12 months in the absence of disease progression or unacceptable toxicity. In case the participant receives clinical benefit from the treatment, treatment can proceed up to 24 months.

experimental: Arm C: ONC206 + radiation therapy, DMGs with evidence of first progression but previously untreated

Participants undergo standard of care radiation therapy daily 5 days a week and receive ONC206 PO up to six times per week. Cycles repeat every 28 days for up to 12 months in the absence of disease progression or unacceptable toxicity. In case the participant receives clinical benefit from the treatment, treatment can proceed up to 24 months.

experimental: Arm D: ONC206 Therapy, Primary malignant CNS tumors with progression

Participants receive ONC206 PO once a day (QD) up to six times per week. Cycles repeat every 28 days for up to 12 months in the absence of disease progression or unacceptable toxicity. In case the participant receives clinical benefit from the treatment, treatment can proceed up to 24 months.

Interventions

ONC206

Given orally (PO)

Standard of Care Radiation Therapy

Undergo RT

Optional Proton (1H) MR spectroscopy (MRS)

Optional imaging procedure

Primary outcome measure

  • Proportion of participants with dose-limiting toxicities (DLT) [ Time Frame: Within 4 weeks after first dose ]
  • Maximum tolerated dose (MTD) of ONC206 [ Time Frame: Within 4 weeks after first dose ]

Central Contacts and Locations

Central contacts

PNOC Operations

(415) 502-1600PNOC023@ucsf.edu

Locations

University of California, San Francisco

Recruiting

San Francisco, California, United States, 94143

Contacts

PNOC Operations

415-502-1600PNOC023@ucsf.edu

PNOC023@ucsf.edu

Principal Investigator:

Sabine Mueller, MD, PhD

Children's National Hospital

Recruiting

Washington D.C., District of Columbia, United States, 20010

Contacts

Lindsay Kilburn, MD

202-476-2800lkilburn@childrensnational.org

Principal Investigator:

Lindsay Kilburn, MD

Emory University

Recruiting

Atlanta, Georgia, United States, 30322

Contacts

Tobey MacDonald, MD

tobey.macdonald@emory.edu

University of Michigan

Recruiting

Ann Arbor, Michigan, United States, 48109

Contacts

Carl Koschmann, MD

CCPBTC-CRA@med.umich.edu

Children's Hospital of Philadelphia

Recruiting

Philadelphia, Pennsylvania, United States, 19104

Contacts

Cassie Kline, MD

215-590-2299klinec@chop.edu

Principal Investigator:

Cassie Kline, MD

More Information

Sponsor

Sabine Mueller, MD, PhD

Last update posted

Jun 11, 2026

Last verified

Jun, 2026

Keywords

  • H3 K27M-Mutant

Trial information was received from ClinicalTrials.gov and was last updated on 2026-09-18. This information was provided to ClinicalTrials.gov by Sabine Mueller, MD, PhD on 2026-06-11.