Recruiting

Anabolic Sensitivity

Sponsor:

University of Toronto

Code:

NCT06209424

Conditions

Resistance Exercise

Physical Inactivity

Amino Acids

Dietary Protein

Eligibility Criteria

Sex: All

Age: 18 - 35

Healthy Volunteers: Accepted

Interventions

Habitual Activity

Step-Reduction

Resistance Exercise

Study Details

Brief summary:

Developing tools to detect when our bodies are more resistant towards protein synthesis is valuable for identification of when someone may be at risk of losing body or muscle mass such as with aging or certain diseases. The current study aims to refine our previous breath test method to be more effective at measuring changes in how the body processes protein in different situations, such as resting, reducing physical activity, and doing resistance exercise. We hypothesize that using a lower amount of dietary amino acids in our breath test will be effective at detecting lower amounts of amino acids used after exercise, and a greater amount with step reduction compared to normal activity levels

Conditions

Resistance Exercise

Physical Inactivity

Amino Acids

Dietary Protein

Study ID

NCT06209424

Start date

Jan 15, 2024

Status verified date

Jan, 2024

Completion date

Apr 30, 2025

Anticipated

Primary completion date

Dec 31, 2024

Anticipated

Eligibility Criteria

Eligibility Criteria

Sex: All

Age: 18 - 35

Healthy Volunteers: Accepted

Inclusion Criteria:

  • Healthy young (age: 18-35 years)
  • BMI between normal to overweight (18.5-29.9 kg/m2)
  • if oral contraceptive (OC) user, must be on monophasic OCs for at least 3 months prior to study
  • if non-OC user, then must have regular menstrual cycles (length: 25-35 days) for at least 3 months prior to study and at least 6 months off of OCs

Exclusion Criteria:

  • Chronic disease diagnosis (cardiovascular, thyroid, diabetes)
  • Current or recent remission of cancer
  • Regular use of NSAID (except low-dose aspirin), anticoagulants
  • Use of prescription drugs that would impact muscle protein synthesis (e.g., Statins, Lithium, ADHD medication, etc..)
  • Insertion of intrauterine device (IUD) - exception: copper
  • Smoking
  • Use of illicit drugs (growth hormones, testosterone)

Study Design

Enrollment

12 participants

Anticipated

Allocation

Randomized

Intervention Model

Crossover

Primary purpose

Other

Interventions and Outcome Measures

Arms

active comparator: In-Person Phase

Habitual Activity + Resistance Exercise Metabolic Trials

active comparator: At-Home Phase

Habitual Activity + Step-Reduction Metabolic Trials

Interventions

Habitual Activity

Participants will maintain habitual levels of physical activity (inclusive of structured physical activity).

Step-Reduction

Participants will be required to reduce their daily step-counts to <2,000 steps/day. Further, they will be required to refrain from structured physical activity.

Resistance Exercise

Participants will undergo a 50-minute resistance exercise protocol, which includes multiple sets of different exercises using weights. Each set will consist of 10 repetitions at 75% of their 1 repetition maximum (1RM). The exercises include bench press superset with lat pulldowns, overhead press superset with seated cable rows, leg press, and leg extensions with 90s rest in between sets. Before the exercise protocol, there will be a standardized 10-minute warm-up that involves cycling, leg swings, arm circles, bodyweight squats, and bench push-ups

Primary outcome measure

  • Exogenous Leucine Oxidation (umol/kg) [ Time Frame: 5 hours ]
  • Net Leucine Retention (umol/kg) [ Time Frame: 5 hours ]

Central Contacts and Locations

Central contacts

Daniel R Moore, PhD

4169464088dr.moore@utoronto.ca

Hugo JW Fung, PhD (c)

6472098764hugojernwai.fung@utoronto.ca

Locations

Goldring Centre for High Performance Sport at the University of Toronto

Recruiting

Toronto, Ontario, Canada, M5S2C9

Contacts

Daniel R Moore, PhD

416464088dr.moore@utoronto.ca

Hugo JW Fung, PhD (c)

6472098764hugojernwai.fung@utoronto.ca

More Information

Sponsor

University of Toronto

Last update posted

Jan 17, 2024

Last verified

Jan, 2024

Keywords

  • Sex
  • Step-Reduction
  • Habitual Activity
  • Protein Metabolism
  • Resistance Exercise
  • Stable Isotopes
  • Anabolic Sensitivity

Trial information was received from ClinicalTrials.gov and was last updated on 2026-09-10. This information was provided to ClinicalTrials.gov by University of Toronto on 2024-01-17.