Recruiting

Observational Study

Sponsor:

University of Manitoba

Code:

NCT06677749

Conditions

Skeletal Muscle

Eligibility Criteria

Sex: All

Age: 18 - 35

Healthy Volunteers: Accepted

Interventions

Eccentric resistance-exercise

Study Details

Brief summary:

The purpose of this study is to evaluate the acute local skeletal muscle inflammatory response to thigh muscle (quadriceps) eccentric muscle actions. Eccentric muscle actions occur when the muscle is lengthening as it contracts. This is a pilot project to assess the degree of inflammatory biomarkers, called myokines, that are released locally in skeletal muscle tissue after an acute bout of resistance-exercise using a resistance-exercise machine called an isokinetic dynamometer.

Conditions

Skeletal Muscle

Study ID

NCT06677749

Start date

Nov 15, 2024

Status verified date

Jul, 2024

Completion date

Nov 1, 2025

Anticipated

Primary completion date

Jul 31, 2025

Anticipated

Eligibility Criteria

Eligibility Criteria

Sex: All

Age: 18 - 35

Healthy Volunteers: Accepted

Inclusion Criteria:

  • 18-35 years old
  • healthy
  • recreationally active (>/= 150 minutes of moderate to vigorous physical activity per week for at least the past three months)

Exclusion Criteria:

  • "yes" answer to the Get Active Questionnaire which screens for health conditions that may affect exercise ability

Study Design

Enrollment

12 participants

Anticipated

Intervention Model

Single group

Primary purpose

Basic Science

Interventions and Outcome Measures

Arms

experimental: Eccentric Exercise

300 repetitions of isokinetic dynamometer eccentric knee extensions.

Interventions

Eccentric resistance-exercise

Each participant will complete 300 repetitions of eccentric knee extensions using an isokinetic dynamometer after a baseline skeletal muscle biopsy is taken. Three hours following this, a second muscle biopsy will be taken to evaluate the changes in the dependent variables being measured (myokines).

Primary outcome measure

  • Apelin [ Time Frame: Baseline and 3-hours post-exercise ]
  • Fibroblast growth factor-21 [ Time Frame: Baseline and 3-hours post-exercise ]
  • Irisin [ Time Frame: Baseline and 3-hours post-exercise ]
  • Interleukin-6 [ Time Frame: Baseline and 3-hours post-exercise ]
  • Interleukin-15 [ Time Frame: Baseline and 3-hours post-exercise ]

Central Contacts and Locations

Central contacts

Stephen M Cornish, PhD

204-381-7296Stephen.Cornish@umanitoba.ca

Locations

Applied Research Centre, Faculty of Kinesiology, University of Manitoba

Recruiting

Winnipeg, Manitoba, Canada, R3T2N2

Contacts

Stephen M Cornish, Ph.D.

204-474-9981Stephen.Cornish@umnaitoba.ca

Zach Henderson, M.Sc.

204-474-7858zachariah.henderson@umanitoba.ca

Principal Investigator:

Stephen M Cornish, Ph.D.

More Information

Sponsor

University of Manitoba

Last update posted

Nov 7, 2024

Last verified

Jul, 2024

Trial information was received from ClinicalTrials.gov and was last updated on 2026-09-10. This information was provided to ClinicalTrials.gov by University of Manitoba on 2024-11-07.