Recruiting

Nutritional Quality

Sponsor:

Daniel Moore

Code:

NCT07148908

Conditions

Protein Metabolism

Eligibility Criteria

Sex: All

Age: 18 - 45

Healthy Volunteers: Accepted

Interventions

Yeast Protein Supplement

Whey Protein Isolate Supplement

Collagen Hydrolysate Supplement

Study Details

Brief summary:

Given the relatively high carbon footprint and sustainability of animal-based proteins, there is a growing interest in determining the nutritional quality non-animal-based protein sources. The overall objective of this investigation is to examine the impact of different animal and microbial-based protein sources to support whole body protein synthesis in adults. To do this, investigators will employ a 'breath test' method developed in our laboratory as well as urine sampling. The results of this study will allow us to better understand the impact of dietary protein quality for maintaining health and body protein mass in adults.

Conditions

Protein Metabolism

Study ID

NCT07148908

Start date

Jul 1, 2025

Status verified date

Aug, 2025

Completion date

Dec 31, 2025

Anticipated

Primary completion date

Oct 31, 2025

Anticipated

Eligibility Criteria

Eligibility Criteria

Sex: All

Age: 18 - 45

Healthy Volunteers: Accepted

Inclusion Criteria:

  • Healthy, male and female, recreationally-active participants.
  • Healthy will be defined as screened by the PAR-Q+ (Appendix B) and The Physical Activity Readiness Questionnaire (Appendix C).
  • Participants will be aged 18-45 years old.
  • Participants are willing to abide by the compliance rules of this study.
  • Self-reported regular menstrual cycle (25-35d) within the last 3 months (female participants).
  • Use of monophasic combined oral contraceptives (COC) containing 21 active pills (e.g., Yaz, Marvelon, Cyclen, etc.; the brand of COC will be recorded) (female participants).

Exclusion Criteria:

  • Inability to adhere to any of the compliance rules judged by principal investigator (e.g. dairy protein or yeast allergy).
  • Self-reported regular tobacco use.
  • Self-reported illicit drug use (e.g., growth hormone, testosterone, etc.).
  • Individuals who have participated in studies within the past year involving any of the stable isotopes in the study.
  • Use of multiphasic COCs due to the difficulty of controlling for hormonal fluctuations in such formulations (female participants).
  • Use of COCs containing >21 active pills per cycle due to potential differences in their effect on metabolism (female participants).
  • Use of other forms of hormonal contraception (e.g., progestin-only pill, hormonal IUD, intravaginal ring) (female participants).
  • Not currently or previously (in the past 6 months) on a vegan diet

Study Design

Enrollment

13 participants

Anticipated

Allocation

Randomized

Intervention Model

Crossover

Primary purpose

Basic Science

Interventions and Outcome Measures

Arms

experimental: Yeast Protein Supplement

Participants will be consuming test beverage composed of yeast protein and their whole-body protein metabolism will be determined over the subsequent 8 hours.

active comparator: Whey Protein Isolate Supplement

Participants will be consuming test beverage composed of whey protein isolate and their whole-body protein metabolism will be determined over the subsequent 8 hours.

active comparator: Collagen Hydrolysate Supplement

Participants will be consuming test beverage composed of collagen protein and their whole-body protein metabolism will be determined over the subsequent 8 hours.

Interventions

Yeast Protein Supplement

Each protein will be consumed at 0.9g/kg/d in n=8 hourly drinks

Whey Protein Isolate Supplement

Each protein will be consumed at 0.9g/kg/d in n=8 hourly drinks

Collagen Hydrolysate Supplement

Each protein will be consumed at 0.9g/kg/d in n=8 hourly drinks

Primary outcome measure

  • 1. Phenylalanine excretion (umol/kg/h) [ Time Frame: 8 hours ]
  • Phenylalanine oxidation (umol/kg/h) [ Time Frame: 8 hours ]

Central Contacts and Locations

Central contacts

Daniel R. Moore, Ph.D.

416-946-4088drmoore@utoronto.ca

Locations

Kinesiology & Physical Education

Recruiting

Toronto, Ontario, Canada, M5S 2C9

Contacts

Sabrina T. Barsky, Ph.D.

647-540-0115sabrina.barsky@utoronto.ca

More Information

Sponsor

Daniel Moore

Last update posted

Aug 29, 2025

Last verified

Aug, 2025

Keywords

  • Protein quality
  • protein metabolism
  • stable isotopes
  • protein oxidation
  • indicator amino acid oxidation
  • protein synthesis

Trial information was received from ClinicalTrials.gov and was last updated on 2026-09-10. This information was provided to ClinicalTrials.gov by Daniel Moore on 2025-08-29.