Recruiting
Phase 2

Chemotherapy, Immunotherapy, Targeted Therapy

Sponsor:

American Association for Cancer Research

Code:

NCT07290985

Conditions

Gastroesophageal Adenocarcinoma

Eligibility Criteria

Sex: All

Age: 18+

Healthy Volunteers: Not accepted

Interventions

Tislelizumab

Leucovorin

Oxaliplatin

Fluorouracil

Zanidatamab

Study Details

Brief summary:

This study will test a new personalized treatment approach for patients with stomach or esophageal cancer. It will take place in two stages and aims to find the best combination of chemotherapy, immunotherapy, and targeted drugs based on each patient's tumor biomarkers.

Upon enrollment onto the study, patients will consent to tumor biomarker testing and may receive one cycle of standard chemotherapy while awaiting results. Those with a matching biomarker will join the corresponding treatment group that combines chemotherapy, an immune checkpoint inhibitor, and/or a targeted therapy. In Stage I of the study, treatment lasts about four months before surgery, followed by an additional eight months of therapy for a total of one year.

The most effective treatments from Stage I will be studied further in Stage II of the study to see whether some patients can safely avoid surgery. Those patients enrolled during Stage II will receive four months of the same combination treatment (chemotherapy, an immune checkpoint inhibitor, and/or a targeted therapy) but may be eligible to skip surgery if their cancer completely disappears after pre-surgery therapy. All patients will then receive an additional eight months of therapy and those who skipped surgery will be closely monitored with scans and endoscopies.

Conditions

Gastroesophageal Adenocarcinoma

Study ID

NCT07290985

Start date

Jul 20, 2026

Status verified date

Jul, 2026

Completion date

Dec 1, 2028

Anticipated

Primary completion date

Jun 1, 2028

Anticipated

Eligibility Criteria

Eligibility Criteria

Sex: All

Age: 18+

Healthy Volunteers: Not accepted

Inclusion Criteria:

  • Histologically confirmed, resectable adenocarcinoma of the stomach, esophagus, or gastroesophageal junction (Stage II or higher, T2N0 with high-risk features).
  • Complete surgical resection deemed achievable by multidisciplinary evaluation.
  • Willingness to undergo tumor biopsies for biomarker analysis (HER2, FGFR2b, PD-L1, MSI) at screening, progression, or pre/post- surgery.
  • Life expectancy ≥ 24 weeks. Eastern Cooperative Oncology Group (ECOG) performance status ≤1.
  • Adequate organ function:

  • Hematologic: absolute neutrophil count (ANC) ≥1.5 ×10⁹/L, platelets ≥100 ×10⁹/L, hemoglobin ≥ 8 g/dL
  • Hepatic: aspartate aminotransferase (AST)/ alanine aminotransferase (ALT) ≤2.5 × ULN, total bilirubin ≤1.5 × upper limit of normal (ULN) (≤2.5 × ULN for Gilbert's)
  • Renal: creatinine ≤1.5 × ULN or creatinine clearance ≥50 mL/min/1.73 m²
  • Willingness for blood samples to be drawn for research purposes.
  • Baseline dihydropyrimidine dehydrogenase (DPD) testing per local guidelines; dosing of 5-FU adjusted for deficiency.
  • Use of two effective contraception methods for women of childbearing potential and men during and 4 months after study; pregnant or breastfeeding women excluded.
  • Must have the ability to understand and the willingness to sign a written informed consent document.
  • Willingness and able to comply with the protocol for the duration of the study, including attending scheduled visits, examinations, the screening procedure, and having their tumor and blood molecularly characterized.
  • Understands they must meet all Inclusion and Exclusion criteria in the sub-protocol for the treatment for which they will be later assigned

Additional inclusion criteria for all sub-studies:

• FFPE tumor sample tested at a central laboratory confirming PD-L1 Tumor Area Positivity (TAP) score ≥1%.

Additional inclusion criteria specific to HER2 sub-study:

  • Histologically confirmed diagnosis of resectable (i.e., radical surgery eligible), HER2-positive (defined as 3+ HER2 expression by IHC or 2+ HER2 expression by immunohistochemistry (IHC) with in situ hybridization (ISH)-positivity per central assessment) adenocarcinoma of the stomach or esophagus, including the gastroesophageal junction.
  • Formalin-fixed, paraffin-embedded (FFPE) tumor sample tested at a central laboratory confirming HER2-positive status.
  • Left ventricular ejection fraction (LVEF) ≥50% as determined by either echocardiogram or multiple gated acquisition scan (MUGA).

Exclusion criteria:

  • Unresectable disease, peritoneal dissemination, and/or positive cytology on laparoscopy.
  • Peripheral neuropathy ≥ Grade 2.
  • Active infection.
  • Chronic growth factor support for white blood cells or granulocytes.
  • Concurrent anti-cancer therapy (exceptions: supportive care medications ≥1 month prior, low molecular weight heparin, prior adjuvant hormonal therapy >3 years, palliative radiation

≤14 days prior).
  • Local/systemic therapy for current gastroesophageal diagnosis (except one FLOX/ mFOLFOX6 dose during screening).
  • Significant medical conditions compromising safety or protocol compliance.
  • Preexisting cardiac conditions.
  • Stroke, transient ischemic attack (TIA), or myocardial infarction within 6 months.
  • Prior FGFR-targeted therapy.
  • Prior HER2-targeted therapy (except >5 years prior for breast cancer).
  • Prior checkpoint inhibitor therapy (anti-PD-1/PD- L1/PD-L2).
  • Conditions preventing safe surgery/biopsy.
  • Malabsorption syndrome or inability to swallow oral medication.
  • Pregnant or nursing women
  • History of stem cell or organ transplant.
  • Major surgery within 14 days.
  • Other malignancy within 3 years (exceptions: basal/squamous cell carcinoma, in situ malignancy, low-risk prostate cancer).
  • Systemic corticosteroids >10 mg prednisone/ day or immunosuppressive therapy ≤14 days prior (exceptions: adrenal replacement, topical/ inhaled, short prophylactic courses).
  • HIV with CD4 <350, active hepatitis B or C.
  • Active or relapsing autoimmune disease (exceptions: controlled type I diabetes, hypothyroidism on replacement, controlled celiac disease, mild skin disease, other non- recurrent diseases).

Additional exclusion criteria for all sub-studies:

  • History of hypersensitivity or contraindications to any active substance/active ingredient of any study medication, including chemotherapy components monoclonal antibodies, recombinant proteins, and/or any of the excipients listed in the ingredients of any drug formulation
  • Poorly controlled seizures
  • Clinically significant bleeding (CTCAE ≥ Grade 3) from the gastrointestinal (GI) tract within 4 weeks prior to enrollment.
  • Administered a live vaccine ≤ 4 weeks prior to enrollment
  • History of interstitial lung disease or non-infectious pneumonitis, or with severe dyspnea at rest or requiring supplementary oxygen therapy.
  • Treated with another investigational product within 28 days of enrolment.

Exclusion criteria specific to HER2 sub-study:

  • Total lifetime anthracycline load exceeding 360 mg/m² of doxorubicin or equivalent
  • QTc Fridericia (QTcF) >470 ms. Note: For subjects with longer QTcF on initial electrocardiogram (ECG), follow-up ECG may be performed in triplicate to determine eligibility (e.g., after correction of electrolyte abnormalities, or discontinuation of QT-prolonging drugs).
  • Ongoing Grade 2 or greater diarrhea

Study Design

Enrollment

242 participants

Anticipated

Allocation

Non randomized

Intervention Model

Single group

Primary purpose

Treatment

Interventions and Outcome Measures

Arms

experimental: Zanidatamab + Tislelizumab (HER2) Sub-Study

Patients will receive zanidatamab on Day 1 of each 14-day cycle, followed by tislelizumab and mFOLFOX6 chemotherapy: leucovorin (Day 1), oxaliplatin (Day 1), and 5-FU as a 48-hour continuous intravenous infusion (Days 1-2). Up to 8 preoperative cycles will be given, followed by surgery or organ preservation if a complete response is achieved. Post-surgery or organ preservation, zanidatamab + tislelizumab will continue every 2 weeks for up to 1 year of perioperative therapy.

experimental: Zolbetuximab + Tislelizumab (CLDN18.2) Sub-Study

Patients will receive zolbetuximab on Day 1 of each 14-day cycle, followed by tislelizumab and mFOLFOX6 chemotherapy: leucovorin (Day 1), oxaliplatin (Day 1), and 5-FU as a 48-hour continuous intravenous infusion (Days 1-2). Up to 8 preoperative cycles will be given, followed by surgery or organ preservation if a complete response is achieved. Post-surgery or organ preservation, zolbetuximab + tislelizumab will continue every 2 weeks for up to 1 year of perioperative therapy.

Interventions

Tislelizumab

150 mg every 2 weeks for 4 months pre-operatively and up to 8 months post-operatively

Leucovorin

400 mg/m² every 2 weeks for 4 months pre-operatively

Oxaliplatin

85 mg/m² every 2 weeks for 4 months pre-operatively

Fluorouracil

2,400 mg/m² every 2 weeks for 4 months pre-operatively

Zanidatamab

1,200 mg for patients weighing less than 70 kg and 1,600 mg for patients weighing 70 kg or more every 2 weeks for 4 months pre-operatively and up to 8 months post-operatively

Zolbetuximab

A loading dose of 800mg/m2 zolbetuximab will be administered at cycle 1, followed by subsequent doses of 400 mg/m2 every 2 weeks for 4 months pre-operatively and up to 8 months post-operatively. In the postoperative maintenance therapy phase, dosing of 600 mg/m2 every 3 weeks may also be used

Primary outcome measure

  • Pathological Complete Response (pathCR) Rate (Stage I). [ Time Frame: At time of surgery. ]
  • Organ Preservation Rate (Stage II) [ Time Frame: Up to 12 months post-treatment. ]

Central Contacts and Locations

Central contacts

Locations

University of California, Los Angeles

Recruiting

Los Angeles, California, United States, 90095

Yale University

Recruiting

New Haven, Connecticut, United States, 06520

Princess Margaret Cancer Centre

Recruiting

Toronto, Ontario, Canada

More Information

Sponsor

American Association for Cancer Research

Last update posted

Jul 23, 2026

Last verified

Jul, 2026

Trial information was received from ClinicalTrials.gov and was last updated on 2026-09-09. This information was provided to ClinicalTrials.gov by American Association for Cancer Research on 2026-07-23.